The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
IRS-1 (Phospho-Ser312) Antibody
Synonyms
Insulin receptor substrate-1; IRS-1; IRS1
Immunogen
The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser312.
Immunogen Range
279-328
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000
Specificity
IRS-1 (Phospho-Ser312) Antibody detects endogenous levels of IRS-1 only when phosphorylated at Ser312.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
IRS1
Database Links
Gene ID: 3667 Swiss-Prot #: P35568
OMIM #: 125853/147545 Unigene #: Hs.471508
Target Modification
Phospho
Modification Sites
H:S312 M:S307 R:S307
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using IRS-1 (Phospho-Ser312) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HepG2 cells, using IRS-1 (Phospho-Ser312) Antibody. The lane on the right is blocked with the phospho peptide.