The antiserum was produced against synthesized peptide derived from human HSF1 around the phosphorylation site of Ser303.
Immunogen Range
270-319
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
HSF1 (Phospho-Ser303) Antibody detects endogenous levels of HSF1 only when phosphorylated at Ser303.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
HSF1
Database Links
Gene ID: 3297 Swiss-Prot #: Q00613
OMIM #: 140580 Unigene #: Hs.530227/Hs.571397
Target Modification
Phospho
Modification Sites
H:S303 M:S303
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSF1 (Phospho-Ser303) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from MCF7 cells treated with TNF-alpha 20ng/ml 30', using HSF1 (Phospho-Ser303) Antibody. The lane on the left is blocked with the phospho peptide.