The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
FKHR (Phospho-Ser256) Antibody
Synonyms
FKHR; forkhead box O1A (FKHR); Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO1
Immunogen
The antiserum was produced against synthesized peptide derived from human FKHR around the phosphorylation site of Ser256.
Immunogen Range
223-272
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
FKHR (Phospho-Ser256) Antibody detects endogenous levels of FKHR only when phosphorylated at Ser256.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
FOXO1
Database Links
Gene ID: 2308 Swiss-Prot #: Q12778
OMIM #: 136533/268220 Unigene #: Hs.370666
Target Modification
Phospho
Modification Sites
H:S256 M:S253
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FKHR (Phospho-Ser256) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HeLa cells treated with EGF+Serum, using FKHR (Phospho-Ser256) Antibody. The lane on the right is blocked with the phospho peptide.