The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Ezrin (Phospho-Tyr353) Antibody
Synonyms
Cytovillin; EZRI; p81; VIL2; Villin 2
Immunogen
The antiserum was produced against synthesized peptide derived from human Ezrin around the phosphorylation site of Tyr353.
Immunogen Range
321-370
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
Ezrin (Phospho-Tyr353) Antibody detects endogenous levels of Ezrin only when phosphorylated at Tyr353.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
EZR
Database Links
Gene ID: 7430 Swiss-Prot #: P15311
OMIM #: 123900 Unigene #: Hs.487027
Target Modification
Phospho
Modification Sites
H:Y354 M:Y354 R:Y354
Application Images
Image 1
Western blot analysis of lysates from A431 cells treated with EGF 200ng/ml 30', using Ezrin (Phospho-Tyr353) Antibody. The lane on the right is blocked with the phospho peptide.