The antiserum was produced against synthesized peptide derived from human p44/42 MAP Kinase around the phosphorylation site of Thr202.
Immunogen Range
169-218
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
p44/42 MAP Kinase (Phospho-Thr202) Antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at Thr202.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAPK3
Database Links
Gene ID: 5595/5594 Swiss-Prot #: P27361/P28482
OMIM #: 601795/176948 Unigene #: Hs.861/Hs.431850
Target Modification
Phospho
Modification Sites
H:T202/185 M:T203/183 R:T203/183
Application Images
Image 1
Western blot analysis of lysates from NIH/3T3 cells treated with IFN 2500U/ml 30', using p44/42 MAP Kinase (Phospho-Thr202) Antibody. The lane on the right is blocked with the phospho peptide.