DARPP-32 (Phospho-Thr34) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at Thr34.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PPP1R1B
Database Links
Gene ID: 84152 Swiss-Prot #: Q9UD71
OMIM #: 604399 Unigene #: Hs.286192
Target Modification
Phospho
Modification Sites
H:T34 M:T34 R:T34
Application Images
Image 1
Immunofluorescence analysis of HepG2 cells, using DARPP-32 (Phospho-Thr34) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human brain, using DARPP-32 (Phospho-Thr34) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using DARPP-32 (Phospho-Thr34) Antibody. The lane on the right is blocked with the phospho peptide.