The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
CREB (Phospho-Ser133) Antibody
Synonyms
cAMP responsive element binding protein 1; cAMP-response element binding protein; CREB-1; CREB1
Immunogen
The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser133.
Immunogen Range
100-149
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
CREB (Phospho-Ser133) Antibody detects endogenous levels of CREB only when phosphorylated at Ser133.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CREB (Phospho-Ser133) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from Jurkat and 293 cells treated with UV, using CREB (Phospho-Ser133) Antibody. The lane on the right is blocked with the phospho peptide.