The antiserum was produced against synthesized peptide derived from human Catenin-beta around the phosphorylation site of Ser37.
Immunogen Range
Mar-52
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
Catenin-beta (Phospho-Ser37) Antibody detects endogenous levels of Catenin-beta only when phosphorylated at Ser37.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CTNNB1
Database Links
Gene ID: 1499 Swiss-Prot #: P35222
OMIM #: 116806/132600/155255 Unigene #: Hs.476018
Target Modification
Phospho
Modification Sites
H:S37 M:S37 R:S37
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Catenin-beta (Phospho-Ser37) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells, using Catenin-beta (Phospho-Ser37) Antibody. The lane on the right is blocked with the phospho peptide.