The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
ATF2 (Phospho-Thr71 or 53) Antibody
Synonyms
Activating 2; ATF2; cAMP response element binding protein CRE- BP1; CREB2; CREBP1; Cyclic-AMP-dependent ATF-2; HB16; MXBP protein
Immunogen
The antiserum was produced against synthesized peptide derived from human ATF2 around the phosphorylation site of Thr71 or 53.
Immunogen Range
38-87
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
ATF2 (Phospho-Thr71 or 53) Antibody detects endogenous levels of ATF2 only when phosphorylated at Thr71 or 53.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ATF2
Database Links
Gene ID: 1386 Swiss-Prot #: P15336
OMIM #: 123811 Unigene #: Hs.592510
Target Modification
Phospho
Modification Sites
H:T71 M:T53 R:T53
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ATF2 (Phospho-Thr71 or 53) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HeLa cells treated with UV, using ATF2 (Phospho-Thr71 or 53) Antibody. The lane on the right is blocked with the phospho peptide.