The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Akt2 (Phospho-Ser474) Antibody
Synonyms
kinase Akt2; PKB beta; Protein kinase Akt-2; Protein kinase B, beta; RAC-beta serine/threonine protein kinase; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2
Immunogen
The antiserum was produced against synthesized peptide derived from human Akt2 around the phosphorylation site of Ser474.
Immunogen Range
432-481
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
Akt2 (Phospho-Ser474) Antibody detects endogenous levels of Akt2 only when phosphorylated at Ser474.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
AKT2
Database Links
Gene ID: 208 Swiss-Prot #: P31751
OMIM #: 164731 Unigene #: Hs.631535
Target Modification
Phospho
Modification Sites
H:S474 M:S474 R:S474
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Akt2 (Phospho-Ser474) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using Akt2 (Phospho-Ser474) Antibody. The lane on the right is blocked with the phospho peptide.