The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
4E-BP1 (Phospho-Thr45) Antibody
Synonyms
4EBP1; EIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Insulin-stimulated EIF-4E binding protein PHAS-I; P/OKCL.6; PHAS-1; PHAS-I
Immunogen
The antiserum was produced against synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Thr45.
Immunogen Range
13-62
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
4E-BP1 (Phospho-Thr45) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr45.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
EIF4EBP1
Database Links
Gene ID: 1978 Swiss-Prot #: Q13541
OMIM #: 602223 Unigene #: Hs.411641
Target Modification
Phospho
Modification Sites
H:T46 M:T45 R:T45
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from MDA-MB-435 cells treated with EGF 200ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is blocked with the phospho peptide.