Description
TheExpiFectamine?293TransfectionKitisacorecomponentoftheExpi293?ExpressionSystem.ItisdesignedfortransienttransfectionofhighdensityculturesofHEK(humanembryonickidney)293cells.Thehigh-efficiency,cationic,lipid-basedtransfectionreagentandtransfectionenhancersaredesignedtopowerthehighestposs
IBLelevelofproteinexpressionfromExpi293F?cellsculturedinExpi293?ExpressionMedium?
Designedspecificallyfortransfectionofhighdensitys
USPensioncellculture,withmatchingtransfectionenhancersthatboosttransfectionperformanceandproteinexpression?
Achievesproteinyields2-to10-foldhigherthanothertransfectionreagentsusedonhighdensity293cellcultures?
Employsthesametransientexpressionprotocolstypicallyusedincurrentlow-density293suspensionculturesystemstoeasilyswitchfromlowdensitysystemstothehighyield,highdensityExpi293?ExpressionSystem?
Providesrobustandreproducibletransfectionresults,givingusersgreaterconfidenceintheirresults?
Scaletransfectionsforculturevolumesoflessthan1mLtogreaterthan10liters,whilemaintainingequivalentvolumetricproteinyieldsTheExpiFectamine?293TransfectionKitisspecificallydesignedtoworkwithExpi293F?cellsintheExpi293?ExpressionSystem.Thetransfectionkitconsistsofthecationiclipid-basedExpiFectamine?293ReagentandExpiFectamine?293TransfectionEnhancers1and2.Theenhancersareadded16to18hourspost-transfectiontoboosttransfection,cellvi
ABIlity,andproteinexpression.TheprotocolforuseofExpiFectamine?293Transfectioncomponentsisverysimilartotransfectionprotocolsforothertransientexpressionreagents,suchas293fectinandFreeStyle?MAX.
ForResearchUseOnly.Notforuseindiagnosticprocedures.