The antiserum was produced against synthesized peptide derived from human Syntaxin 1A around the phosphorylation site of Ser14.
Immunogen Range
Jan-50
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Specificity
Syntaxin 1A (Phospho-Ser14) Antibody detects endogenous levels of Syntaxin 1A only when phosphorylated at Ser14.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
STX1A
Database Links
Gene ID: 6804 Swiss-Prot #: Q16623
OMIM #: 186590 Unigene #: Hs.647024
Target Modification
Phospho
Modification Sites
H:S14 M:S14 R:S14
Application Images
Image 1
Immunofluorescence analysis of NIH/3T3 cells, using Syntaxin 1A (Phospho-Ser14) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells, using Syntaxin 1A (Phospho-Ser14) Antibody. The lane on the right is blocked with the phospho peptide.