The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser226.
Immunogen Range
201-250
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Specificity
HSP90B (Phospho-Ser226) Antibody detects endogenous levels of HSP90B only when phosphorylated at Ser226.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
HSP90AB1
Database Links
Gene ID: 3326 Swiss-Prot #: P08238
OMIM #: 140572 Unigene #: Hs.509736/Hs.689683
Target Modification
Phospho
Modification Sites
H:S226 M:S226 R:S226
Application Images
Image 1
Immunofluorescence analysis of NIH/3T3 cells, using HSP90B (Phospho-Ser226) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from A549 cells treated with serum 20% 15', using HSP90B (Phospho-Ser226) Antibody. The lane on the right is blocked with the phospho peptide.