The antiserum was produced against synthesized peptide derived from human S1P Receptor EDG1 around the phosphorylation site of Thr236.
Immunogen Range
206-255
Clonality
Polyclonal
Application Dilutions
IF: 1:100~1:500 ELISA: 1:10000
Specificity
S1P Receptor EDG1 (Phospho-Thr236) Antibody detects endogenous levels of S1P Receptor EDG1 only when phosphorylated at Thr236.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
S1PR1
Database Links
Gene ID: 1901 Swiss-Prot #: P21453
OMIM #: 601974 Unigene #: Hs.154210
Target Modification
Phospho
Modification Sites
H:T236 M:T236 R:T237
Application Images
Image 1
Immunofluorescence analysis of COS7 cells, using S1P Receptor EDG1 (Phospho-Thr236) Antibody. The picture on the right is blocked with the phospho peptide.