The antiserum was produced against synthesized peptide derived from human ROMK/Kir1.1 around the phosphorylation site of Ser44/25.
Immunogen Range
Nov-60
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
Specificity
ROMK/Kir1.1 (Phospho-Ser44/25) Antibody detects endogenous levels of ROMK/Kir1.1 only when phosphorylated at Ser44/25.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
KCNJ1
Database Links
Gene ID: 3758 Swiss-Prot #: P48048
OMIM #: 241200/600359 Unigene #: Hs.527830
Target Modification
Phospho
Modification Sites
H:S44 M:S25 R:S44
Application Images
Image 1
Immunofluorescence analysis of A549 cells, using ROMK/Kir1.1 (Phospho-Ser44/25) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human brain, using ROMK/Kir1.1 (Phospho-Ser44/25) Antibody. The picture on the right is blocked with the phospho peptide.