The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
RAD52 (Phospho-Tyr104) Antibody
Synonyms
DNA repair protein RAD52 homolog; RA52
Immunogen
The antiserum was produced against synthesized peptide derived from human RAD52 around the phosphorylation site of Tyr104.
Immunogen Range
70-119
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
RAD52 (Phospho-Tyr104) Antibody detects endogenous levels of RAD52 only when phosphorylated at Tyr104.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RAD52
Database Links
Gene ID: 5893 Swiss-Prot #: P43351
OMIM #: 600392 Unigene #: Hs.709202
Target Modification
Phospho
Modification Sites
H:Y104 M:Y105
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human testis, using RAD52 (Phospho-Tyr104) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30', using RAD52 (Phospho-Tyr104) Antibody. The lane on the right is blocked with the phospho peptide.