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A11008/eBioscience/Alexa Fluor? 488 Goat Anti-Rabbit IgG (H+L) Antibody
  • A11008/eBioscience/Alexa Fluor? 488 Goat Anti-Rabbit IgG (H+L) Antibody

A11008/eBioscience/Alexa Fluor? 488 Goat Anti-Rabbit IgG (H+L) Antibody

價格: ￥1875.72 市場價: 3126.20
貨號: A11008
品牌: eBioscience
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    • RabbitIgG(H+L)Cross-AdsorbedSecondaryAntibody(A-11008)inIF

      ImmunofluorescenceanalysisofGoatanti-RabbitIgG(H+L)Cross-AdsorbedSecondaryAntibodyAlexaFluor?488conjugatewasperformedusingHeLacellsstainedwithalphaTubulinRabbitPolyclonalAntibody(Product#PA5-16891).Thecellswerefixedwith4%paraformaldehydefor10minutes,permeABIlizedwith0.1%Triton?X-100for10minutes,blockedwith1%BSAfor1hourandlabeledwith2μg/mlRabbitprimaryantibodyfor3hoursatroomtemperature.Goatanti-RabbitIgG(H+L)Cross-AdsorbedSecondaryAntibodyAlexaFluor?488conjugate(Product#A-11008)wasusedataconcentrationof4μg/mlinphosphatebufferedsalinecontaining0.2%BSAfor45minutesatroomtemperature,fordetectionofalphaTubulininthecytoplasm(Panela:green).Nuclei(Panelb:blue)werestainedwithDAPIinSlowFade?GoldAntifadeMountant(Product#S36938).F-actinwasstainedwithRhodaminePhalloidin(Product#R415,1:300)(Panelc:red).Paneldrepresentsthecompositeimage.Nononspecificstainingwasobservedwiththesecondaryantibodyalone(panelf),orwithanisotypecontrol(panele).Theimageswerecapturedat60Xmagnification.

      ProductDetails

      TESTEDAPPLICATIONSDILUTION
      FlowCytometry(Flow)1-10μg/mL
      Immunocytochemistry(ICC)4μg/mL
      Immunofluorescence(IF)4μg/mL
      PUBLISHEDAPPLICATIONS
      Immunohistochemistry(Frozen)(IHC(F))See20publicationsbelow
      Immunohistochemistry(IHC)See20publicationsbelow
      Immunocytochemistry(ICC)See26publicationsbelow
      Immunohistochemistry(Paraffin)(IHC(P))See8publicationsbelow
      FlowCytometry(Flow)See2publicationsbelow
      WesternBlot(WB)See3publicationsbelow
      Immunohistochemistry-FreeFloating(IHC(Free))See1publicationsbelow
      ChIPassay(ChIP)See1publicationsbelow
      MiscellaneousPubMed(MISC)See472publicationsbelow
      SpeciesreactivityRabbit
      Host/IsotypeGoat/IgG
      ClassPolyclonal
      TypeSecondaryAntibody
      ImmunogenGammaImmunoglobinsHeavyandLightchains
      ConjugateAlexaFluor?488
      Excitation/EmissionProfileViewspectra 
      Formliquid
      Concentration2mg/ml
      Purificationpurified
      StoragebufferPBS,pH7.5
      Contains5mMsodiumazide
      Storageconditions4°C,storeindark
      RRIDAB_143165
      TargetIgG
      CrossAdsorptionAgainsthumanIgG,humanserum,mouseIgG,mouseserumandbovineserum
      AntibodyFormWholeAntibody

      ProductSpecificInformation

      AlexaFluordyesareamongthemosttrustedfluorescentdyesavailabletoday.Invitrogen?AlexaFluor?488dyeisabright,green-fluorescentdyewithexcitationideallysuitedtothe488nmlaserline.Forstablesignalgenerationinimagingandflowcytometry,AlexaFluor488dyeispH-insensitiveoverawidemolarrange.Probeswithhighfluorescencequantumyieldandhighphotostabilityallowdetectionoflow-abundanceBIOLOGicalstructureswithgreatsensitivity.AlexaFluor488dyemoleculescanbeattachedtoproteinsathighmolarratioswithoutsignificantself-quenching,enablingbrighterconjugatesandmoresensitivedetection.Thedegreeoflabelingforeachconjugateistypically2-8fluorophoremoleculesperIgGmolecule;theexactdegreeoflabelingisindicatedonthecertificateofanalysisforeachproductlot.

      Thegoatanti-rabbitIgGwholeantibodyconjugatesaremostcommonlypreparedbyimmunizingthehostanimalwithapooledpopulationofimmunoglobulinsfromthetargetspeciesandcanbefurtherpurifiedandmodified(e.g.,immunoaffinitychromatography,antibodyfragmentation,labelconjugation,etc.)togeneratehighlyspecificreagents.Inthefirstroundofpurification,wholeimmunoglobulinsbindingtotheimmunizingantibodyarerecoveredandmainlyconsistofthe~150-kDaIgGclass.FurtherpurificationwithProteinAorGremovesallimmunoglobulinclassesexceptIgGsuchthattheaffinity-purifiedantibodiesreactwithIgGheavychainsandallclassesofimmunoglobulinlightchainsfromrabbit.Tominimizecross-reactivity,thesegoatanti-rabbitwholeantibodieshavebeencross-adsorbedagainsthumanIgG,humanserum,mouseIgG,mouseserum,andbovineserum.Cross-adsorptionorpre-adsorptionisapurificationsteptoincreasespecificityoftheantibodyresultinginhighersensitivityandlessbackgroundstaining.Thesecondaryantibodysolutionispassedthroughacolumnmatrixcontainingimmobilizedserumproteinsfrompotentiallycross-reactivespecies.Onlythenonspecific-bindingsecondaryantibodiesarecapturedinthecolumn,andthehighlyspecificsecondariesflowthrough.Thebenefitsofthisextrastepareapparentinmultiplexing/multicolor-stainingexperimentswherethereispotentialcross-reactivitywithotherprimaryantibodiesorinimmunohistochemistryexperimentswheretherearemaybethepresenceofendogenousimmunoglobulins.Forahighlycross-adsorbedsecondaryantibodyequivalent(orequivalentsecondaryantibodypreparation),pleaseseeproductcatalognumber:A11034.

      Usingconjugatesolutions:Centrifugetheproteinconjugatesolutionbrieflyinamicrocentrifugebeforeuse;addonlythesupernatanttotheexperiment.Thisstepwilleliminateanyproteinaggregatesthatmayhaveformedduringstorage,therebyreducingnonspecificbackgroundstaining.Becausestainingprotocolsvarywithapplication,theappropriatedilutionofantibodyshouldbedeterminedempirically.Forthefluorophore-labeledantibodiesafinalconcentrationof1-10μg/mLshouldbesatisfactoryformostimmunohistochemistryandflowcytometryapplications.

      Background/TargetInformation

      WeofferanextensivelineofInvitrogen?secondaryantibodyconjugateswithwell-characterizedspecificityandlabeledwithawideselectionofpremiumfluorescentdyes,includingInvitrogen?AlexaFluor?fluorescentdyes.Fluorescentsecondaryantibodyconjugatesareusefulinthedetection,sorting,orpurificationofitsspecifiedtargetandidealforfluorescencemicroscopyandconfocallaserscanningmicroscopy,flowcytometry,andfluorescentwesterndetection.ThebreadthoffluorescentMarkersweofferallowsourreagentstobetailoredtoalmostanyfluorescentdetectionsystem.

      Secondaryantibodiesmaybeprovidedinthreeformats:wholeIgG,divalentF(ab')2fragments,andmonovalentFabfragments.Becauseofthehighdegreeofconservationinthestructureofmanyimmunoglobulindomains,mostclass-specificsecondaryantibodiesmustbeaffinity-purifiedandcross-adsorbedtoachieveminimalcross-reactionwithotherimmunoglobulins.

      ForResearchUseOnly.Notforuseindiagnosticprocedures.Notforresalewithoutexpressauthorization.
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