The antiserum was produced against synthesized peptide derived from human IL-7R/CD127 around the phosphorylation site of Tyr449.
Immunogen Range
410-459
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Specificity
IL-7R/CD127 (Phospho-Tyr449) Antibody detects endogenous levels of IL-7R/CD127 only when phosphorylated at Tyr449.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunofluorescence analysis of HUVEC cells, using IL-7R/CD127 (Phospho-Tyr449) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COLO205 cells, using IL-7R/CD127 (Phospho-Tyr449) Antibody. The lane on the right is blocked with the phospho peptide.