The antiserum was produced against synthesized peptide derived from human APC1 around the phosphorylation site of Ser688.
Immunogen Range
654-703
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
Specificity
APC1 (Phospho-Ser688) Antibody detects endogenous levels of APC1 only when phosphorylated at Ser688.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ANAPC1
Database Links
Gene ID: 64682 Swiss-Prot #: Q9H1A4
OMIM #: 608473 Unigene #: Hs.436527
Target Modification
Phospho
Modification Sites
H:S688 M:S688 R:S688
Application Images
Image 1
Immunofluorescence analysis of COS7 cells, using APC1 (Phospho-Ser688) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using APC1 (Phospho-Ser688) Antibody. The picture on the right is blocked with the phospho peptide.