The antiserum was produced against synthesized peptide derived from human PDGFR alpha around the phosphorylation site of Tyr754.
Immunogen Range
721-770
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:5000
Specificity
PDGFR alpha (Phospho-Tyr754) Antibody detects endogenous levels of PDGFR alpha only when phosphorylated at Tyr754.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PGFRA
Database Links
Gene ID: 5156 Swiss-Prot #: P16234
OMIM #: 173490/607685 Unigene #: Hs.74615
Target Modification
Phospho
Modification Sites
H:Y754 M:Y754 R:Y753
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using PDGFR alpha (Phospho-Tyr754) Antibody. The picture on the right is blocked with the phospho peptide.