The antiserum was produced against synthesized peptide derived from human p27 Kip1 around the phosphorylation site of Ser178.
Immunogen Range
144-193
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:10000
Specificity
p27 Kip1 (Phospho-Ser178) Antibody detects endogenous levels of p27 Kip1 only when phosphorylated at Ser178.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.