The antiserum was produced against synthesized peptide derived from human NMDAR2A/B around the phosphorylation site of Tyr1246/1252.
Immunogen Range
1216-1265
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
Specificity
NMDAR2A/B (Phospho-Tyr1246/1252) Antibody detects endogenous levels of NMDAR2A/B only when phosphorylated at Tyr1246/1252.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunofluorescence analysis of HUVEC cells, using NMDAR2A/B (Phospho-Tyr1246/1252) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human brain, using NMDAR2A/B (Phospho-Tyr1246/1252) Antibody. The picture on the right is blocked with the phospho peptide.