Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | HDAC3(Phospho-Ser424)Antibody |
Synonyms | HD3;Histonedeacetylase3;RPD3-2;SMAP45 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanHDAC3aroundthephosphorylationsiteofSer424. |
ImmunogenRange | 379-428 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:1000 |
Specificity | HDAC3(Phospho-Ser424)AntibodydetectsendogenouslevelsofHDAC3onlywhenphosphorylatedatSer424. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | HDAC3 |
DatabaseLinks | GeneID: 8841 Swiss-Prot#: O15379 OMIM#: 605166 Unigene#: Hs.519632 |
TargetModification | Phospho |
ModificationSites | H:S424M:S424R:S424 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofA549cells,usingHDAC3(Phospho-Ser424)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | Immunohistochemistryanalysisofparaffin-embeddedhumanskin,usingHDAC3(Phospho-Ser424)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image3 | WesternblotanalysisoflysatesfromNIH/3T3cells,usingHDAC3(Phospho-Ser424)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |