The antiserum was produced against synthesized peptide derived from human GluR4 around the phosphorylation site of Ser862.
Immunogen Range
828-877
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
GluR4 (Phospho-Ser862) Antibody detects endogenous levels of GluR4 only when phosphorylated at Ser862.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
GRIA4
Database Links
Gene ID: 2893 Swiss-Prot #: P48058
OMIM #: 138246 Unigene #: Hs.503743
Target Modification
Phospho
Modification Sites
H:S862 M:S862 R:S862
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using GluR4 (Phospho-Ser862) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HepG2 cells treated with Forskolin 40nM 30', using GluR4 (Phospho-Ser862) Antibody. The lane on the right is blocked with the phospho peptide.