The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
C-RAF (Phospho-Ser43) Antibody
Synonyms
C-RAF; C-Raf; CRAF; kinase Raf1; Protein kinase raf 1; RAF proto-oncogene serine/threonine-protein kinase; Raf proto-oncogene serine/threonine-protein kinase; RAF-1; Raf-1; RAF1; Similar to murine leukemia viral (V-raf-1) oncogene homolog 1
Immunogen
The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser43.
Immunogen Range
Nov-60
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:5000
Specificity
C-RAF (Phospho-Ser43) Antibody detects endogenous levels of C-RAF only when phosphorylated at Ser43.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RAF1
Database Links
Gene ID: 5894 Swiss-Prot #: P04049
OMIM #: 164760/611553/611554 Unigene #: Hs.159130
Target Modification
Phospho
Modification Sites
H:S43 M:S43 R:S43
Application Images
Image 1
Western blot analysis of lysates from NIH/3T3 cells, using C-RAF (Phospho-Ser43) Antibody. The lane on the right is blocked with the phospho peptide.