Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | Keratin8(Phospho-Ser432)Antibody |
Synonyms | CK8;CK8;CYK8;CytokeratinendoA;K2C8;K8;KRT2-8;KRT8;Keratin,typeIIcytoskeletal8;cytokeratin8 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanKeratin8aroundthephosphorylationsiteofSer432. |
ImmunogenRange | 401-450 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:40000 |
Specificity | Keratin8(Phospho-Ser432)AntibodydetectsendogenouslevelsofKeratin8onlywhenphosphorylatedatSer432. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | KRT8 |
DatabaseLinks | GeneID: 3856 Swiss-Prot#: P05787 OMIM#: 148060/215600 Unigene#: Hs.533782/Hs.708445 |
TargetModification | Phospho |
ModificationSites | H:S432M:S438R:S432 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofHeLacells,usingKeratin8(Phospho-Ser432)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | Immunohistochemistryanalysisofparaffin-embeddedhumancoloncarcinoma,usingKeratin8(Phospho-Ser432)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image3 | Westernblotanalysisoflysatesfrom293cellstreatedwithEGF200ng/ml30",usingKeratin8(Phospho-Ser432)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |