The antiserum was produced against synthesized peptide derived from human CD5 around the phosphorylation site of Tyr453.
Immunogen Range
421-470
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000
Specificity
CD5 (Phospho-Tyr453) Antibody detects endogenous levels of CD5 only when phosphorylated at Tyr453.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CD5
Database Links
Gene ID: 921 Swiss-Prot #: P06127
OMIM #: 153340 Unigene #: Hs.58685
Target Modification
Phospho
Modification Sites
H:Y453 M:Y452 R:Y449
Application Images
Image 1
Immunofluorescence analysis of HepG2 cells, using CD5 (Phospho-Tyr453) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30' , using CD5 (Phospho-Tyr453) Antibody. The lane on the right is blocked with the phospho peptide.