The antiserum was produced against synthesized peptide derived from human CD4 around the phosphorylation site of Ser433.
Immunogen Range
401-450
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity
CD4 (Phospho-Ser433) Antibody detects endogenous levels of CD4 only when phosphorylated at Ser433.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CD4
Database Links
Gene ID: 920 Swiss-Prot #: P01730
OMIM #: 186940 Unigene #: Hs.631659
Target Modification
Phospho
Modification Sites
H:S433 M:S432
Application Images
Image 1
Immunofluorescence analysis of HepG2 cells, using CD4 (Phospho-Ser433) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human brain, using CD4 (Phospho-Ser433) Antibody. The picture on the right is blocked with the phospho peptide.