The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
PKC zeta (Phospho-Thr560) Antibody
Synonyms
KPCZ; PKC-zeta; PKC2; PRKCZ; Protein kinase C zeta type; kinase PKC-zeta; nPKC-zeta
Immunogen
The antiserum was produced against synthesized peptide derived from human PKC zeta around the phosphorylation site of Thr560.
Immunogen Range
526-575
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
PKC zeta (Phospho-Thr560) Antibody detects endogenous levels of PKC zeta only when phosphorylated at Thr560.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PRKCZ
Database Links
Gene ID: 5590 Swiss-Prot #: Q05513
OMIM #: 176982 Unigene #: Hs.496255
Target Modification
Phospho
Modification Sites
H:T560 M:T560 R:T560
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using PKC zeta (Phospho-Thr560) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using PKC zeta (Phospho-Thr560) Antibody. The lane on the right is blocked with the phospho peptide.