The antiserum was produced against synthesized peptide derived from human PKC epsilon around the phosphorylation site of Ser729.
Immunogen Range
688-737
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
PKC epsilon (Phospho-Ser729) Antibody detects endogenous levels of PKC epsilon only when phosphorylated at Ser729.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PRKCE
Database Links
Gene ID: 5581 Swiss-Prot #: Q02156
OMIM #: 176975 Unigene #: Hs.580351
Target Modification
Phospho
Modification Sites
H:S729 M:S729 R:S729
Application Images
Image 1
Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using PKC epsilon (Phospho-Ser729) Antibody. The lane on the right is blocked with the phospho peptide.