Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | PKCalpha(Phospho-Tyr657)Antibody |
Synonyms | kinasePKC-alpha;KPCA;PKCIII;PKC-A;PKC-alpha;PKC-III;PRKACA;PRKCA;ProteinkinaseC,alphatype |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanPKCalphaaroundthephosphorylationsiteofTyr657. |
ImmunogenRange | 623-672 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000ELISA:1:1000 |
Specificity | PKCalpha(Phospho-Tyr657)AntibodydetectsendogenouslevelsofPKCalphaonlywhenphosphorylatedatTyr657. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | PRKCA |
DatabaseLinks | GeneID: 5578 Swiss-Prot#: P17252 OMIM#: 176960 Unigene#: Hs.531704/Hs.708867 |
TargetModification | Phospho |
ModificationSites | H:Y657M:Y657R:Y657 |
ApplicationImages |
Image1 | WesternblotanalysisoflysatesfromCOLO205cells,usingPKCalpha(Phospho-Tyr657)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |