The antiserum was produced against synthesized peptide derived from human Chk2 around the phosphorylation site of Thr383.
Immunogen Range
356-405
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000
Specificity
Chk2 (Phospho-Thr383) Antibody detects endogenous levels of Chk2 only when phosphorylated at Thr383.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunofluorescence analysis of HeLa cells, using Chk2 (Phospho-Thr383) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with UV 30', using Chk2 (Phospho-Thr383) Antibody. The lane on the right is blocked with the phospho peptide.