The antiserum was produced against synthesized peptide derived from human PFKFB2 around the phosphorylation site of Ser483.
Immunogen Range
451-500
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
PFKFB2 (Phospho-Ser483) Antibody detects endogenous levels of PFKFB2 only when phosphorylated at Ser483.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
F262
Database Links
Gene ID: 5208 Swiss-Prot #: O60825
OMIM #: 171835 Unigene #: Hs.282702
Target Modification
Phospho
Modification Sites
H:S483 M:S486 R:S486
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PFKFB2 (Phospho-Ser483) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with Heat shock, using PFKFB2 (Phospho-Ser483) Antibody. The lane on the right is blocked with the phospho peptide.