The antiserum was produced against synthesized peptide derived from human Pin1 around the phosphorylation site of Ser16.
Immunogen Range
Jan-50
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
Pin1 (Phospho-Ser16) Antibody detects endogenous levels of Pin1 only when phosphorylated at Ser16.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PIN1
Database Links
Gene ID: 5300 Swiss-Prot #: Q13526
OMIM #: 601052 Unigene #: Hs.465849
Target Modification
Phospho
Modification Sites
H:S16 M:S16 R:S16
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human heart, using Pin1 (Phospho-Ser16) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with insulin 0.01U/ml 15', using Pin1 (Phospho-Ser16) Antibody. The lane on the right is blocked with the phospho peptide.