The antiserum was produced against synthesized peptide derived from human PAK2 around the phosphorylation site of Ser20.
Immunogen Range
May-54
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
PAK2 (Phospho-Ser20) Antibody detects endogenous levels of PAK2 only when phosphorylated at Ser20.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PAK2
Database Links
Gene ID: 5062 Swiss-Prot #: Q13177
OMIM #: 605022 Unigene #: Hs.518530
Target Modification
Phospho
Modification Sites
H:S20 M:S20 R:S20
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PAK2 (Phospho-Ser20) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with Sorbitol 0.4M 30', using PAK2 (Phospho-Ser20) Antibody. The lane on the right is blocked with the phospho peptide.