The antiserum was produced against synthesized peptide derived from human p40 phox around the phosphorylation site of Thr154.
Immunogen Range
120-169
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:1000
Specificity
p40 phox (Phospho-Thr154) Antibody detects endogenous levels of p40 phox only when phosphorylated at Thr154.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NCF4
Database Links
Gene ID: 4689 Swiss-Prot #: Q15080
OMIM #: 601488 Unigene #: Hs.474781
Target Modification
Phospho
Modification Sites
H:T154 M:T154
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human ovary, using p40 phox (Phospho-Thr154) Antibody. The picture on the right is blocked with the phospho peptide.