The antiserum was produced against synthesized peptide derived from human LKB1 around the phosphorylation site of Thr189.
Immunogen Range
155-204
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
LKB1 (Phospho-Thr189) Antibody detects endogenous levels of LKB1 only when phosphorylated at Thr189.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
STK11
Database Links
Gene ID: 6794 Swiss-Prot #: Q15831
OMIM #: 175200/273300/602216 Unigene #: Hs.515005
Target Modification
Phospho
Modification Sites
H:T189 M:T189
Application Images
Image 1
Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using LKB1 (Phospho-Thr189) Antibody. The lane on the right is blocked with the phospho peptide.