The antiserum was produced against synthesized peptide derived from human TFII-I around the phosphorylation site of Tyr248.
Immunogen Range
214-263
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:5000
Specificity
TFII-I (Phospho-Tyr248) Antibody detects endogenous levels of TFII-I only when phosphorylated at Tyr248.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
GTF2I
Database Links
Gene ID: 2969 Swiss-Prot #: P78347
OMIM #: 194050/601679 Unigene #: Hs.647041
Target Modification
Phospho
Modification Sites
H:Y248 M:Y248 R:Y248
Application Images
Image 1
Western blot analysis of lysates from LOVO cells, using TFII-I (Phospho-Tyr248) Antibody. The lane on the right is blocked with the phospho peptide.