The antiserum was produced against synthesized peptide derived from human Survivin around the phosphorylation site of Thr34.
Immunogen Range
Nov-60
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
Survivin (Phospho-Thr34) Antibody detects endogenous levels of Survivin only when phosphorylated at Thr34.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BIRC5
Database Links
Gene ID: 332 Swiss-Prot #: O15392
OMIM #: 603352 Unigene #: Hs.514527
Target Modification
Phospho
Modification Sites
H:T34 M:T34 R:T34
Application Images
Image 1
Western blot analysis of lysates from HT29 cells treated with EGF 100ug/ml 30', using Survivin (Phospho-Thr34) Antibody. The lane on the right is blocked with the phospho peptide.