The antiserum was produced against synthesized peptide derived from human PECAM-1 around the phosphorylation site of Tyr713.
Immunogen Range
686-735
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000
Specificity
PECAM-1 (Phospho-Tyr713) Antibody detects endogenous levels of PECAM-1 only when phosphorylated at Tyr713.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PDGFRA
Database Links
Gene ID: 5175 Swiss-Prot #: P16284
OMIM #: 173445 Unigene #: Hs.514412/Hs.709189
Target Modification
Phospho
Modification Sites
H:Y713 M:Y702
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using PECAM-1 (Phospho-Tyr713) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HepG2 cells, using PECAM-1 (Phospho-Tyr713) Antibody. The lane on the right is blocked with the phospho peptide.