The antiserum was produced against synthesized peptide derived from human p90 RSK around the phosphorylation site of Thr359 and Ser363.
Immunogen Range
331-380
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
p90 RSK (Phospho-Thr359+Ser363) Antibody detects endogenous levels of p90 RSK only when phosphorylated at Thr359 and Ser363.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RPS6KA1
Database Links
Gene ID: 6195 Swiss-Prot #: Q15418
OMIM #: 601684 Unigene #: Hs.149957
Target Modification
Phospho
Modification Sites
H:T359/S363 M:S352/T348 R:S363/T359
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p90 RSK (Phospho-Thr359+Ser363) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using p90 RSK (Phospho-Thr359+Ser363) Antibody. The lane on the right is blocked with the phospho peptide.