The antiserum was produced against synthesized peptide derived from human IKK-beta around the phosphorylation site of Tyr199.
Immunogen Range
166-215
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
IKK-beta (Phospho-Tyr199) Antibody detects endogenous levels of IKK-beta only when phosphorylated at Tyr199.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
IKBKB
Database Links
Gene ID: 3551 Swiss-Prot #: O14920
OMIM #: 603258 Unigene #: Hs.656458
Target Modification
Phospho
Modification Sites
H:Y199 M:Y199 R:Y199
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IKK-beta (Phospho-Tyr199) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml+Calyculin A 50nM 5', using IKK-beta (Phospho-Tyr199) Antibody. The lane on the right is blocked with the phospho peptide.