The antiserum was produced against synthesized peptide derived from human FLT3 around the phosphorylation site of Tyr599.
Immunogen Range
565-614
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
FLT3 (Phospho-Tyr599) Antibody detects endogenous levels of FLT3 only when phosphorylated at Tyr599.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
FLT3
Database Links
Gene ID: 2322 Swiss-Prot #: P36888
OMIM #: 136351 Unigene #: Hs.507590
Target Modification
Phospho
Modification Sites
H:Y599 M:Y600
Application Images
Image 1
Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using FLT3 (Phospho-Tyr599) Antibody. The lane on the right is blocked with the phospho peptide.