Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | EstrogenReceptor-alpha(Phospho-Tyr537)Antibody |
Synonyms | ER;ESR;ESR1;ESTR;ESTRA;EstrADIolreceptor;Estrogenreceptor;NR3A1 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanEstrogenReceptor-alphaaroundthephosphorylationsiteofTyr537. |
ImmunogenRange | 501-550 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:1000 |
Specificity | EstrogenReceptor-alpha(Phospho-Tyr537)AntibodydetectsendogenouslevelsofEstrogenReceptor-alphaonlywhenphosphorylatedatTyr537. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | ESR1 |
DatabaseLinks | GeneID: 2099 Swiss-Prot#: P03372 OMIM#: 133430 Unigene#: Hs.208124 |
TargetModification | Phospho |
ModificationSites | H:Y537M:Y541M:Y542 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanlungcarcinoma,usingEstrogenReceptor-alpha(Phospho-Tyr537)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | Westernblotanalysisoflysatesfrom293cells,usingEstrogenReceptor-alpha(Phospho-Tyr537)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |