The antiserum was produced against synthesized peptide derived from human CD3 zeta around the phosphorylation site of Tyr142.
Immunogen Range
111-160
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
CD3 zeta (Phospho-Tyr142) Antibody detects endogenous levels of CD3 zeta only when phosphorylated at Tyr142.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CD247
Database Links
Gene ID: 919 Swiss-Prot #: P20963
OMIM #: 186780/610163 Unigene #: Hs.156445
Target Modification
Phospho
Modification Sites
H:Y142 M:Y142 R:Y142
Application Images
Image 1
Western blot analysis of lysates from Jurkat cells treated with UV 15', using CD3 zeta (Phospho-Tyr142) Antibody. The lane on the right is blocked with the phospho peptide.