The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
CBL (Phospho-Tyr674) Antibody
Synonyms
Cas-Br-M (murine) ecotropic retroviral transforming; CBL E3 ubiquitin protein ligase; CBL2; Proto-oncogene c-CBL; Signal transduction protein CBL
Immunogen
The antiserum was produced against synthesized peptide derived from human CBL around the phosphorylation site of Tyr674.
Immunogen Range
640-689
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
CBL (Phospho-Tyr674) Antibody detects endogenous levels of CBL only when phosphorylated at Tyr674.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CBL
Database Links
Gene ID: 867 Swiss-Prot #: P22681
OMIM #: 165360 Unigene #: Hs.504096
Target Modification
Phospho
Modification Sites
H:Y674 M:Y672 R:Y780
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using CBL (Phospho-Tyr674) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HepG2 cells treated with Na2VO3 0.3nM 40', using CBL (Phospho-Tyr674) Antibody. The lane on the right is blocked with the phospho peptide.