The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
E2F1 (Phospho-Thr433) Antibody
Synonyms
E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; RBBP3; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Transcription factor E2F1
Immunogen
The antiserum was produced against synthesized peptide derived from human E2F1 around the phosphorylation site of Thr433.
Immunogen Range
388-437
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:5000
Specificity
E2F1 (Phospho-Thr433) Antibody detects endogenous levels of E2F1 only when phosphorylated at Thr433.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
E2F1
Database Links
Gene ID: 1869 Swiss-Prot #: Q01094
OMIM #: 189971 Unigene #: Hs.654393
Target Modification
Phospho
Modification Sites
H:T433 M:T426
Application Images
Image 1
Western blot analysis of lysates from HeLa cells treated with Etoposide 25uM 24h, using E2F1 (Phospho-Thr433) Antibody. The lane on the right is blocked with the phospho peptide.