The antiserum was produced against synthesized peptide derived from human Cyclin D1 around the phosphorylation site of Thr286.
Immunogen Range
246-295
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
Cyclin D1 (Phospho-Thr286) Antibody detects endogenous levels of Cyclin D1 only when phosphorylated at Thr286.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CCND1
Database Links
Gene ID: 595 Swiss-Prot #: P24385
OMIM #: 168461/254500 Unigene #: Hs.523852
Target Modification
Phospho
Modification Sites
H:T286 M:T286 R:T286
Application Images
Image 1
Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using Cyclin D1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.