The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
CDC6 (Phospho-Ser54) Antibody
Synonyms
CDC18; CDC6 cell division cycle 6 homolog; CDC6-related protein
Immunogen
The antiserum was produced against synthesized peptide derived from human CDC6 around the phosphorylation site of Ser54.
Immunogen Range
20-69
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
CDC6 (Phospho-Ser54) Antibody detects endogenous levels of CDC6 only when phosphorylated at Ser54.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CDC6
Database Links
Gene ID: 990 Swiss-Prot #: Q99741
OMIM #: 602627 Unigene #: Hs.405958
Target Modification
Phospho
Modification Sites
H:S54 M:S55
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using CDC6 (Phospho-Ser54) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using CDC6 (Phospho-Ser54) Antibody. The lane on the right is blocked with the phospho peptide.